Grants and collaborations
Development of reagents for a diagnostic method using rapid polymerase reaction 2020-1.1.2-PIACI-KFI-2021-00274
Isothermal chain reaction (LAMP) is a DNA synthesis method, in which the DNA strand is copied at a constant temperature (60-65 ˚C). The LAMP reaction can even be performed in a water bath. The resulting product can be detected by colorimetric or fluorescent methods. Applying LAMP in diagnostic procedures, a ’yes / no’ response can be obtained in as little as 40-60 minutes. Such a measurement method has already been developed for the detection of several pathogens, such as coronavirus. Our proposal is to develop reagents that can reliably identify a virus or bacterium in 20-30 minutes, even in a district physician’s office, using the specificity of the polymerase reaction.
Development of flagellin-based subunit vaccines against corona viruses 2020-2.1.1-ED-2020-00013
In connection with the fight against the coronavirus epidemic, we want to develop a new type of protein-based vaccine that elicits an innate immune response and an acquired immunity, thus making our body resistant to the attack of the real virus. The idea is to combine two proteins in the vaccine: one is the part of the flagellin protein, the main component of the locomotion organelle of the salmonella bacterium, which has been shown to be a useful carrier or excipient to increase the effectiveness of vaccines. The other component of the fusion protein would be the cell surface-binding portion of the “spike” protein responsible for recognizing the target cell on the surface of selected animal coronaviruses. It is believed that by incorporating the receptor-binding domain of the coronavirus, which directs entry into infected cells, inside the flagellin, there is a promising potential for the production of an effective vaccine.
Successfully completed grants:
Development of novel Hot-Start (HS) polymerase technologies for the Polymerase Chain Reaction (PCR) (NEMZ_15-1-2016-0001, E!9360-NEWHS4PCR)
The goal of the project was to create a new quality class of HS PCR reagents and qPCR mixes. The project has accomplished with great success and the products developed are now available on the market world-wide. The project has combined the skill sets of two SMEs, the Polymerase Chain Reaction (PCR) reagents development (PCR Biosystems) and protein biochemistry (TargetEx).
The aim of this collaboration is to develop novel ON-type gene expression systems based on the currently available, OFF-type E-Rex and PEACE systems, where the term “ON-type” means that gene expression is switched on in the presence, and off in the absence of the relevant drugs (erythromycin, phloretin). Turning these OFF-type systems into ON-type (i.e. achieving reverse molecules) also includes and requires development of a novel computational modelling methodology for such systems. Further aim is to improve the recently developed, already ON-type RuX system (regulated by RU486). With the international SwitchItOn consortium, a combination of three SMEs and an academia, we have combined the needed expertise and knowledge to bring novel switches for the 2nd generation of models of human disease to the market. The project has a website (http://switchiton.eu/) and runs until Q2 of 2021 by plans.
The goal of the project is to develop and make manufacture-ready a novel, innovative, market-ready, ISO certified RNase inhibitor that is often used in research and human diagnostics. For this, we will produce a protein that is more stable than the marketed inhibitors due to particular changes in the amino acid composition. Besides, we are planning to develop a buffer system that increases the stability of the protein. The project runs until end of 2020 by plans.